Staphylococcus aureus PCR Pusa Su'esu'ega

Fa'amatalaga Puupuu:

Staphylococcus aureus e a'afia i le ituaiga Staphylococcus ma o se siama kalama lelei.Ose mea'ai masani e feavea'i pathogenic microorganism e mafai ona maua enterotoxins ma mafua ai meaʻai oona.

Auina imeli ia matou

Fa'amatalaga Oloa

Faailoga o oloa

Igoa Oloa

Staphylococcus aureus Pusa Su'esu'ega PCR (lyophilized)

Tele

48su'ega/kit, 50su'ega/kit

Fa'aoga Fa'amoemoe

Staphylococcus aureus e a'afia i le ituaiga Staphylococcus ma o se siama kalama lelei.Ose mea'ai masani e feavea'i pathogenic microorganism e mafai ona maua enterotoxins ma mafua ai meaʻai oona.O lenei pusa o loʻo faʻaaogaina le faʻavae o le PCR fluorescent taimi moni ma e talafeagai mo le suʻesuʻeina lelei o le Staphylococcus aureus i meaʻai, vai faʻataʻitaʻiga, feces, vomit, faʻatamaoaiga vai ma isi faʻataʻitaʻiga.

O mea o lo'o iai

Vaega	afifi	fa'amatalaga	Mea'ai
Fa'afefiloi PCR	1 × fagu (Lyophilized pauta)	50Su'ega	dNTPs, MgCl₂, Ulua'i, Su'esu'ega, Taq DNA polymerase
Fa'afefiloi PCR	6×0.2ml 8 pai-sese paipa(Lyophilized)	48Su'ega	dNTPs, MgCl₂, Ulua'i, Su'esu'ega, Taq DNA polymerase
Pulea lelei	1*0.2ml paipa (lyophilized)	10 Su'ega	Plasmid po'o le Pseudovirus o lo'o iai ni vaega fa'apitoa
Fa'amavae fofo	1.5 ml Cryotube	500uL	/
Pule Leaga	1.5 ml Cryotube	100uL	0.9%NaCl

Teuina & Ola Fa'asa

(1) E mafai ona feaveaʻi le pusa ile vevela potu.

(2) O le fata ola e 18 masina ile -20℃ ma le 12 masina ile 2℃~30℃.

(3) Va'ai le igoa i luga o le pusa mo le aso gaosiga ma le aso e muta ai.

(4) E tatau ona teuina le lyophilized powder version reagent i le -20 ℃ pe a maeʻa le faʻamavae ma le toe faʻamaisaina - e tatau ona itiiti ifo i le 4 taimi.

Meafaigaluega

GENECHECKER UF-150, UF-300 mea faigaluega PCR fluorescence i le taimi moni.

Fa'asologa o Galuega

a) Faiga fagu:

b) 8 lelei-sese paipa lomiga:

Pcr Amplification

Fautuaga Fautuaina

Laa	Ta'amilosaga	vevela (℃)	Taimi	Auala fa'alelei
1	1	95	2min	/
2	40	95	6s	/
2	40	60	12s	Aoina le fa'amalama a le FAM

Fa'amatala I'uga o Su'ega

Channel	Fa'amatalaga o taunu'uga
FAMChannel	Fa'amatalaga o taunu'uga
Ct≤35	Staphylococcus aureus Lelei
Undet	Staphylococcus aureus Le lelei
35<Ct≤40	Masalotoe fai,toe su'e*

*Afaio le toe su'e iuga ole ala ole FAMhasa Ct tau ≤40ma faʻaalia masani“S”si'i fa'alautele foliga, o le taunuuga e faauigaina o se mea lelei, a leai e leaga.