E.coli O157: H7 PCR mauaina pusa
Igoa oloa
E.coli O157: H7 PCR mauaina pusa (Lyophilized)
Tele
48test / kit, 50tests / kit
Faʻaaoga Faʻaaoga
Escherichia coli O157: H7 (E.coli O157: H7) o le siama o le kalama-le lelei i le ituaiga Enterobacteriaceae, lea e maua mai ai le tele o vailaʻau oona Vero. I le falemaʻi, e masani lava ona tupu faʻafuaseʻi ma tiga tiga o le manava ma manava suavai, sosoʻo mai ma le manava tata i ni nai aso mulimuli ane, lea e mafai ona oo atu ai i le fiva pe leai se fiva, ma le oti i matuia mataupu. O lenei pusa e fetaui lelei mo le mauaina lelei o le Escherichia coli O157: H7 i meaai, vai faʻataʻitaʻiga, feces, puaʻi, siama-faʻalauteleina suavai ma isi faʻataʻitaʻiga faʻaaogaina le faʻavae o taimi moni PCR. Lyophilized), o loʻo iai le DNA faʻateleina le enzyme, tali faʻafomaʻi, primers faʻapitoa ma suʻesuʻega manaʻomia mo fluorescent PCR mauaina ..
Oloa Aano
| Vaega | Afifi | faʻamatalaga faʻapitoa | Elemeni |
| E.coli O157: H7 PCR Faʻafefiloi | 1 × fagu (Lyophilized paʻu) | 50Tofotofoga | dNTPs, MgCl2, Faʻatomuaga, Masalosalo, Reverse Transcriptase, Taq DNA polymerase |
| 6 × 0.2ml 8 faʻasolosolo lelei fusi paipa(Faʻafilifili) | 48Tofotofoga | ||
| Pulea lelei | 1 * 0.2ml faagaau (lyophilized) | 10 Suʻega |
Plasmid o loʻo iai E.coli O157: H7 vaega faʻapitoa |
| Faʻamamaina fofo | 1.5 ml Cryotube | 500uL | / |
| Leaga Pulea | 1.5 ml Cryotube | 200uL | 0.9% NaCl |
Teuina o Mea & Fata Olaga
(1) E mafai ona ave le pusa i le vevela o le potu.
(2) O le fata olaga o 18 masina i -20 ℃ ma 12 masina i le 2 ℃ ~ 30 ℃.
(3) Vaʻai le igoa faʻaigoa i le pusa mo le aso na faia ai ma le aso faʻamutaina.
(4) O le lyophilized efuefu lomiga reagent tatau ona teuina i -20 ℃ pe a maeʻa ma le toe faʻamalo -thaw tatau ona itiiti ifo i le 4 taimi.
Tufugaaʻao
GENECHECKER UF-150, UF-300 mea moni PC-taimi fluorescence PCR.
Faagaioiga Faagaioiga
a) Fagu faʻamatalaga:
b) 8 lelei-fasi fagu faʻagaau:
Pcr Faʻateleina
Fautuaina Faatulagaina
| Sitepu | Taʻamilosaga | Vevela (℃) | Taimi | Fluorescence auala |
| 1 | 1 | 95 | 2min | |
| 2 | 40 | 95 | 5s | |
| 60 | 10s | Aoina mai le fluorescence a le FAM |
* Faʻaliga: O le faʻailoga o le FAM fluorescence channel o le a aoina i le 60 ℃.
Faʻamatalaina o iʻuga o Suʻega
|
Channel |
Faʻamatalaina o iʻuga |
|
Channel FAM |
|
|
Ct≤35 |
E.coli O157: H7 Mautinoa |
|
Faʻamutaina |
E.coli O157: H7 Le lelei |
|
35<Ct≤40 |
Suspicious resut, retest* |
*If the retest result of FAM channel has a Ct value ≤40 and shows typical “S” shape amplification curve, the result is interpreted as positive, otherwise it is negative.
