E.coli O157: H7 PCR mauaina pusa

Faʻamatalaga puʻupuʻu:

Escherichia coli O157: H7 (E.coli O157: H7) o le siama o le kalama-le lelei i le ituaiga Enterobacteriaceae, lea e maua mai ai le tele o vailaʻau oona Vero.


Oloa Faʻamatalaga

Pine o oloa

Igoa oloa

E.coli O157: H7 PCR mauaina pusa (Lyophilized)

Tele

48test / kit, 50tests / kit

Faʻaaoga Faʻaaoga

Escherichia coli O157: H7 (E.coli O157: H7) o le siama o le kalama-le lelei i le ituaiga Enterobacteriaceae, lea e maua mai ai le tele o vailaʻau oona Vero. I le falemaʻi, e masani lava ona tupu faʻafuaseʻi ma tiga tiga o le manava ma manava suavai, sosoʻo mai ma le manava tata i ni nai aso mulimuli ane, lea e mafai ona oo atu ai i le fiva pe leai se fiva, ma le oti i matuia mataupu. O lenei pusa e fetaui lelei mo le mauaina lelei o le Escherichia coli O157: H7 i meaai, vai faʻataʻitaʻiga, feces, puaʻi, siama-faʻalauteleina suavai ma isi faʻataʻitaʻiga faʻaaogaina le faʻavae o taimi moni PCR. Lyophilized), o loʻo iai le DNA faʻateleina le enzyme, tali faʻafomaʻi, primers faʻapitoa ma suʻesuʻega manaʻomia mo fluorescent PCR mauaina ..

Oloa Aano

Vaega Afifi faʻamatalaga faʻapitoa Elemeni
E.coli O157: H7 PCR Faʻafefiloi 1 × fagu (Lyophilized paʻu)  50Tofotofoga dNTPs, MgCl2, Faʻatomuaga, Masalosalo, Reverse Transcriptase, Taq DNA polymerase
6 × 0.2ml 8 faʻasolosolo lelei fusi paipa(Faʻafilifili) 48Tofotofoga
Pulea lelei 1 * 0.2ml faagaau (lyophilized)  10 Suʻega

Plasmid o loʻo iai E.coli O157: H7 vaega faʻapitoa

Faʻamamaina fofo 1.5 ml Cryotube 500uL /
Leaga Pulea 1.5 ml Cryotube 200uL 0.9% NaCl

Teuina o Mea & Fata Olaga

(1) E mafai ona ave le pusa i le vevela o le potu.

(2) O le fata olaga o 18 masina i -20 ℃ ma 12 masina i le 2 ℃ ~ 30 ℃.

(3) Vaʻai le igoa faʻaigoa i le pusa mo le aso na faia ai ma le aso faʻamutaina.

(4) O le lyophilized efuefu lomiga reagent tatau ona teuina i -20 ℃ pe a maeʻa ma le toe faʻamalo -thaw tatau ona itiiti ifo i le 4 taimi.

Tufugaaʻao

GENECHECKER UF-150, UF-300 mea moni PC-taimi fluorescence PCR.

Faagaioiga Faagaioiga

a) Fagu faʻamatalaga:

1

b) 8 lelei-fasi fagu faʻagaau:

2

Pcr Faʻateleina

Fautuaina Faatulagaina

Sitepu     Taʻamilosaga Vevela (℃)   Taimi Fluorescence auala
1 1 95 2min  
2 40 95 5s  
60 10s Aoina mai le fluorescence a le FAM

* Faʻaliga: O le faʻailoga o le FAM fluorescence channel o le a aoina i le 60 ℃.

Faʻamatalaina o iʻuga o Suʻega

Channel

Faʻamatalaina o iʻuga

Channel FAM

Ct≤35

E.coli O157: H7 Mautinoa

Faʻamutaina

E.coli O157: H7 Le lelei

35<Ct≤40

Suspicious resut, retest*

*If the retest result of FAM channel has a Ct value ≤40 and shows typical “S” shape amplification curve, the result is interpreted as positive, otherwise it is negative.


  • Previous:
  • Next:

  • Related Products