Pseudorabies siama (gB) PCR mauaina pusa

Faʻamatalaga puʻupuʻu:

O lenei ato e faʻaaoga ai metotia masani a le PCR fluorescent e iloa ai le RNA o le Pseudorabies siama (gB gene) (PRV) i mea fai faʻamaʻi o le tino e pei o le tone, lymph nodes ma spleen ma suavai faʻamaʻi mea pei o tui ma toto o puaʻa.


Oloa Faʻamatalaga

Pine o oloa

Igoa oloa

Pseudorabies siama (gB) PCR Detection Kit (Lyophilized)

Tele

48test / kit, 50tests / kit

Faʻaaoga Faʻaaoga

O lenei ato e faʻaaoga ai metotia masani a le PCR fluorescent e iloa ai le RNA o le Pseudorabies siama (gB gene) (PRV) i mea fai faʻamaʻi o le tino e pei o le tone, lymph nodes ma spleen ma suavai faʻamaʻi mea pei o tui ma toto o puaʻa. E talafeagai mo le mauaina, faʻamaonia ma faʻamaʻi suʻesuʻega o le Pseudorabies siama (gB gene). O le ato o le ALL-READY PCR SYSTEM (Lyophilized), o loʻo iai le DNA amplification enzyme, tali faʻafomaʻi, primers faʻapitoa ma suʻesuʻega manaʻomia mo malamalama fluorescent RT-PCR.

Oloa Aano

Vaega Afifi faʻamatalaga faʻapitoa Elemeni
PRV PCR Faʻafefiloi 1 × fagu (Lyophilized paʻu)  50Tofotofoga dNTPs, MgCl2, Faʻatomuaga, Masalo, Taq DNA polymerase
6 × 0.2ml 8 faʻasolosolo lelei fusi paipa(Faʻafilifili) 48Tofotofoga
Pulea lelei 1 * 0.2ml faagaau (lyophilized)  10 Suʻega

Plasmid poʻo Pseudovirus o loʻo iai PRV (gB) vaega ninii

Faʻamamaina fofo 1.5 ml Cryotube 500uL /
Leaga Pulea 1.5 ml Cryotube 200uL 0.9% NaCl

Teuina o Mea & Fata Olaga

(1) E mafai ona ave le pusa i le vevela o le potu.

(2) O le fata olaga o 18 masina i -20 ℃ ma 12 masina i le 2 ℃ ~ 30 ℃.

(3) Vaʻai le igoa faʻaigoa i le pusa mo le aso na faia ai ma le aso faʻamutaina.

(4) O le lyophilized efuefu lomiga reagent tatau ona teuina i -20 ℃ pe a maeʻa ma le toe faʻamalo -thaw tatau ona itiiti ifo i le 4 taimi.

Tufugaaʻao

GENECHECKER UF-150, UF-300 mea moni PC-taimi fluorescence PCR.

Faagaioiga Faagaioiga

a) Fagu faʻamatalaga:

1

b) 8 lelei-fasi fagu faʻagaau:

2

Pcr Faʻateleina

Faatulagaina Fautuaina

Sitepu

Taʻamilosaga

Vevela (℃)

Taimi

Fluorescence auala

1

1

95

2min

/

2

40

95

5s

/

60

10s

Aoina mai le fluorescence a le FAM

* Faʻaliga: O faʻailoga o le FAM fluorescence channel o le a aoina i le 60 ℃.

Faʻamatalaina o iʻuga o Suʻega

Channel

Faʻamatalaina o iʻuga

Channel FAM

Ct≤35

PRV (gB) Lelei

Faʻamutaina

PRV (gB) Le lelei

35<Ct≤40

Suspicious resut, retest*

*If the retest result of FAM channel has a Ct value ≤40 and shows typical “S” shape amplification curve, the result is interpreted as positive, otherwise it is negative.


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