Pseudorabies siama (gB) PCR mauaina pusa
Igoa oloa
Pseudorabies siama (gB) PCR Detection Kit (Lyophilized)
Tele
48test / kit, 50tests / kit
Faʻaaoga Faʻaaoga
O lenei ato e faʻaaoga ai metotia masani a le PCR fluorescent e iloa ai le RNA o le Pseudorabies siama (gB gene) (PRV) i mea fai faʻamaʻi o le tino e pei o le tone, lymph nodes ma spleen ma suavai faʻamaʻi mea pei o tui ma toto o puaʻa. E talafeagai mo le mauaina, faʻamaonia ma faʻamaʻi suʻesuʻega o le Pseudorabies siama (gB gene). O le ato o le ALL-READY PCR SYSTEM (Lyophilized), o loʻo iai le DNA amplification enzyme, tali faʻafomaʻi, primers faʻapitoa ma suʻesuʻega manaʻomia mo malamalama fluorescent RT-PCR.
Oloa Aano
| Vaega | Afifi | faʻamatalaga faʻapitoa | Elemeni |
| PRV PCR Faʻafefiloi | 1 × fagu (Lyophilized paʻu) | 50Tofotofoga | dNTPs, MgCl2, Faʻatomuaga, Masalo, Taq DNA polymerase |
| 6 × 0.2ml 8 faʻasolosolo lelei fusi paipa(Faʻafilifili) | 48Tofotofoga | ||
| Pulea lelei | 1 * 0.2ml faagaau (lyophilized) | 10 Suʻega |
Plasmid poʻo Pseudovirus o loʻo iai PRV (gB) vaega ninii |
| Faʻamamaina fofo | 1.5 ml Cryotube | 500uL | / |
| Leaga Pulea | 1.5 ml Cryotube | 200uL | 0.9% NaCl |
Teuina o Mea & Fata Olaga
(1) E mafai ona ave le pusa i le vevela o le potu.
(2) O le fata olaga o 18 masina i -20 ℃ ma 12 masina i le 2 ℃ ~ 30 ℃.
(3) Vaʻai le igoa faʻaigoa i le pusa mo le aso na faia ai ma le aso faʻamutaina.
(4) O le lyophilized efuefu lomiga reagent tatau ona teuina i -20 ℃ pe a maeʻa ma le toe faʻamalo -thaw tatau ona itiiti ifo i le 4 taimi.
Tufugaaʻao
GENECHECKER UF-150, UF-300 mea moni PC-taimi fluorescence PCR.
Faagaioiga Faagaioiga
a) Fagu faʻamatalaga:
b) 8 lelei-fasi fagu faʻagaau:
Pcr Faʻateleina
Faatulagaina Fautuaina
|
Sitepu |
Taʻamilosaga |
Vevela (℃) |
Taimi |
Fluorescence auala |
|
1 |
1 |
95 |
2min |
/ |
|
2 |
40 |
95 |
5s |
/ |
|
60 |
10s |
Aoina mai le fluorescence a le FAM |
* Faʻaliga: O faʻailoga o le FAM fluorescence channel o le a aoina i le 60 ℃.
Faʻamatalaina o iʻuga o Suʻega
|
Channel |
Faʻamatalaina o iʻuga |
|
Channel FAM |
|
|
Ct≤35 |
PRV (gB) Lelei |
|
Faʻamutaina |
PRV (gB) Le lelei |
|
35<Ct≤40 |
Suspicious resut, retest* |
*If the retest result of FAM channel has a Ct value ≤40 and shows typical “S” shape amplification curve, the result is interpreted as positive, otherwise it is negative.
