Listeria monocytogenes Pusa Su'esu'ega PCR

Fa'amatalaga Puupuu:

Listeria monocytogenes ose microbacterium e mafai ona ola i le va o le 4 ℃ ma le 45 ℃.O se tasi lea o faʻamaʻi autu e lamatia ai le soifua maloloina o tagata i meaʻai pusaaisa.


Fa'amatalaga Oloa

Faailoga o oloa

Igoa Oloa

Listeria monocytogenes Pusa Su'esu'ega PCR

Tele

48su'ega/kit, 50su'ega/kit

Fa'aoga Fa'amoemoe

Listeria monocytogenes ose microbacterium e mafai ona ola i le va o le 4 ℃ ma le 45 ℃.O se tasi lea o faʻamaʻi autu e lamatia ai le soifua maloloina o tagata i meaʻai pusaaisa.O faʻaaliga autu o faʻamaʻi pipisi o septicemia, maningitis ma mononucleosis.O lenei pusa e fetaui lelei mo le suʻesuʻeina lelei o Listeria monocytogenes i meaʻai, vai faʻataʻitaʻiga, feces, vomit, siama-faʻaleleia vai ma isi faʻataʻitaʻiga e faʻaaoga ai le mataupu faavae o le PCR fluorescence taimi moni. O le pusa o se ALL-READY PCR SYSTEM (Lyophilized) , o lo'o i ai le DNA fa'ateleina enzyme, reverse transcriptase, tali fa'apolopolo, fa'amatalaga fa'apitoa ma su'esu'ega e mana'omia mo le su'esu'eina o le PCR fa'alelei.

Teuina & Ola Fa'asa

(1) E mafai ona feaveaʻi le pusa ile vevela potu.

(2) O le fata ola e 18 masina ile -20℃ ma le 12 masina ile 2℃~30℃.

(3) Va'ai le igoa i luga o le pusa mo le aso gaosiga ma le aso e muta ai.

(4) E tatau ona teuina le lyophilized powder version reagent i le -20 ℃ pe a maeʻa le faʻamavae ma le toe faʻamaisaina - e tatau ona itiiti ifo i le 4 taimi.

O mea o lo'o iai

Vaega afifi fa'amatalaga Mea'ai
Listeria monocytogenes PCR Mix 1 × fagu (Lyophilized pauta)  50Su'ega dNTPs, MgCl2, Ulua'i, Su'esu'ega, Reverse Transcriptase, Taq DNA polymerase
6×0.2ml 8 pai-sese paipa(Lyophilized) 48Su'ega
Pulea lelei 1*0.2ml paipa (lyophilized)  10 Su'ega

Plasmid o lo'o iai Listeria monocytogenes vaega fa'apitoa

Fa'amavae fofo 1.5 ml Cryotube 500uL /
Pule Leaga 1.5 ml Cryotube 200uL 0.9%NaCl

Meafaigaluega

GENECHECKER UF-150, UF-300 mea faigaluega PCR fluorescence i le taimi moni.

Fa'asologa o Galuega

a)

1

e)

2

Pcr Amplification

FautuainaFaatulagaina

Laa Ta'amilosaga vevela (℃) Taimi Auala fa'alelei
1 1 50 8min  
2 1 95 2min  
3 40 95 5s  
60 10s Aoina le fa'amalama a le FAM

*Manatua: Ole a aoina fa'ailo ole fa'amalama ole FAM ile 60℃.

Fa'amatala I'uga o Su'ega

Auala

Fa'amatalaga o taunu'uga

FAM Channel

Ct≤35

Listeria monocytogenes Lelei

Undet

Listeria monocytogenes Leaga

35

I'uga masalosalo, toe su'e*

*Afai o le fa'ai'uga toe su'esu'e a le ala FAM e iai le Ct tau ≤40 ma fa'aalia ai le fa'atupu fa'atuputeleina o le "S", o le fa'ai'uga e fa'auiga lelei, a leai e leaga.


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