Porcine Circovirus ituaiga 2 PCR pusa su'esu'e

Fa'amatalaga Puupuu:

O lenei pusa o lo'o fa'aogaina le auala moni o le PCR fluorescent e iloa ai le RNA o le Porcine circovirus type 2 (PCV2) i mea fa'ama'i aano e pei o tonsils, lymph nodes ma mea fa'ama'i fa'ama'i ma vai e pei o tui ma le toto o.


Fa'amatalaga Oloa

Faailoga o oloa

Igoa Oloa

Porcine circovirus type 2 PCR Detection Kit (Lyophilized)

Tele

48su'ega/kit, 50su'ega/kit

Fa'aoga Fa'amoemoe

O lenei pusa o lo'o fa'aogaina le auala moni o le PCR fluorescent e iloa ai le RNA o le Porcine circovirus type 2 (PCV2) i mea fa'ama'i aano e pei o tonsils, lymph nodes ma mea fa'ama'i fa'ama'i ma vai e pei o tui ma le toto o.E talafeagai mo su'esu'ega, su'esu'ega ma su'esu'ega fa'ama'i o le Porcine circovirus type 2. O le pusa o se ALL-READY PCR SYSTEM(Lyophilized), o lo'o i ai le DNA amplification enzyme, reaction buffer, fa'amatalaga fa'apitoa ma su'esu'ega e mana'omia mo le su'esu'eina o le PCR fluorescent. .

O mea o lo'o iai

Vaega afifi fa'amatalaga Mea'ai
Faafefiloi PCV2 PCR 1 × fagu (Lyophilized pauta)  50Su'ega dNTPs, MgCl2, Ulua'i, Su'esu'ega, Taq DNA polymerase
6×0.2ml 8 pai-sese paipa(Lyophilized) 48Su'ega
Pulea lelei 1*0.2ml paipa (lyophilized)  10 Su'ega

Plasmid po'o le Pseudovirus PCV2 o lo'o iai ni vaega fa'apitoa

Fa'amavae fofo 1.5 ml Cryotube 500uL /
Pule Leaga 1.5 ml Cryotube 200uL 0.9%NaCl

Teuina & Ola Fa'asa

(1) E mafai ona feaveaʻi le pusa ile vevela potu.

(2) O le fata ola e 18 masina ile -20℃ ma le 12 masina ile 2℃~30℃.

(3) Va'ai le igoa i luga o le pusa mo le aso gaosiga ma le aso e muta ai.

(4) E tatau ona teuina le lyophilized powder version reagent i le -20 ℃ pe a maeʻa le faʻamavae ma le toe faʻamaisaina - e tatau ona itiiti ifo i le 4 taimi.

Meafaigaluega

GENECHECKER UF-150, UF-300 mea faigaluega PCR fluorescence i le taimi moni.

Fa'asologa o Galuega

a) Faiga fagu:

1

b) 8 lelei-sese paipa lomiga:

2

Pcr Amplification

Fautuaga Fautuaina

Laa

Ta'amilosaga

vevela (℃)

Taimi

Auala fa'alelei

1

1

95

2min

/

2

40

95

5s

/

60

10s

Aoina le fa'amalama a le FAM

*Manatua: Ole a aoina fa'ailo ole fa'amalama ole FAM ile 60℃.

Fa'amatala I'uga o Su'ega

Auala

Fa'amatalaga o taunu'uga

FAM Channel

Ct≤35

Vibrio Parahaemolyticus Lelei

Undet

Vibrio Parahaemolyticus Negative

35

I'uga masalosalo, toe su'e*

*Afai o le fa'ai'uga toe su'esu'e a le ala FAM e iai le Ct tau ≤40 ma fa'aalia ai le fa'atupu fa'atuputeleina o le "S", o le fa'ai'uga e fa'auiga lelei, a leai e leaga.


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