中文Listeria monocytogenes Pusa Su'esu'ega PCR
Igoa Oloa
Listeria monocytogenes Pusa Su'esu'ega PCR
Tele
48su'ega/kit, 50su'ega/kit
Fa'aoga Fa'amoemoe
Listeria monocytogenes ose microbacterium e mafai ona ola i le va o le 4 ℃ ma le 45 ℃.O se tasi lea o faʻamaʻi autu e lamatia ai le soifua maloloina o tagata i meaʻai pusaaisa.O faʻaaliga autu o faʻamaʻi pipisi o septicemia, maningitis ma mononucleosis.O lenei pusa e fetaui lelei mo le suʻesuʻeina lelei o Listeria monocytogenes i meaʻai, vai faʻataʻitaʻiga, feces, vomit, siama-faʻaleleia vai ma isi faʻataʻitaʻiga e faʻaaoga ai le mataupu faavae o le PCR fluorescence taimi moni. O le pusa o se ALL-READY PCR SYSTEM (Lyophilized) , o lo'o i ai le DNA fa'ateleina enzyme, reverse transcriptase, tali fa'apolopolo, fa'amatalaga fa'apitoa ma su'esu'ega e mana'omia mo le su'esu'eina o le PCR fa'alelei.
Teuina & Ola Fa'asa
(1) E mafai ona feaveaʻi le pusa ile vevela potu.
(2) O le fata ola e 18 masina ile -20℃ ma le 12 masina ile 2℃~30℃.
(3) Va'ai le igoa i luga o le pusa mo le aso gaosiga ma le aso e muta ai.
(4) E tatau ona teuina le lyophilized powder version reagent i le -20 ℃ pe a maeʻa le faʻamavae ma le toe faʻamaisaina - e tatau ona itiiti ifo i le 4 taimi.
O mea o lo'o iai
| Vaega | afifi | fa'amatalaga | Mea'ai |
| Listeria monocytogenes PCR Mix | 1 × fagu (Lyophilized pauta) | 50Su'ega | dNTPs, MgCl2, Ulua'i, Su'esu'ega, Reverse Transcriptase, Taq DNA polymerase |
| 6×0.2ml 8 pai-sese paipa(Lyophilized) | 48Su'ega | ||
| Pulea lelei | 1*0.2ml paipa (lyophilized) | 10 Su'ega | Plasmid o lo'o iai Listeria monocytogenes vaega fa'apitoa |
| Fa'amavae fofo | 1.5 ml Cryotube | 500uL | / |
| Pule Leaga | 1.5 ml Cryotube | 200uL | 0.9%NaCl |
Meafaigaluega
GENECHECKER UF-150, UF-300 mea faigaluega PCR fluorescence i le taimi moni.
Fa'asologa o Galuega
a)
e)
Pcr Amplification
FautuainaFaatulagaina
| Laa | Ta'amilosaga | vevela (℃) | Taimi | Auala fa'alelei |
| 1 | 1 | 50 | 8min | |
| 2 | 1 | 95 | 2min | |
| 3 | 40 | 95 | 5s | |
| 60 | 10s | Aoina le fa'amalama a le FAM |
*Manatua: Ole a aoina fa'ailo ole fa'amalama ole FAM ile 60℃.
Fa'amatala I'uga o Su'ega
| Auala | Fa'amatalaga o taunu'uga |
| FAM Channel | |
| Ct≤35 | Listeria monocytogenes Lelei |
| Undet | Listeria monocytogenes Leaga |
| 35 | I'uga masalosalo, toe su'e* |
*Afai o le fa'ai'uga toe su'esu'e a le ala FAM e iai le Ct tau ≤40 ma fa'aalia ai le fa'atupu fa'atuputeleina o le "S", o le fa'ai'uga e fa'auiga lelei, a leai e leaga.
